Nuf2 is required for chromosome segregation during mouse oocyte meiotic maturation |
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| Authors: | Teng Zhang Yang Zhou Shu-Tao Qi Zhen-Bo Wang Wei-Ping Qian Ying-Chun Ouyang Wei Shen Heide Schatten Qing-Yuan Sun |
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| Affiliation: | 1.Institute of Reproductive Sciences; College of Animal Science and Technology; Qingdao Agricultural University; Qingdao, China;2.State Key Laboratory of Reproductive Biology; Institute of Zoology; Chinese Academy of Sciences; Beijing, China;3.Department of Reproductive Medicine; Peking University Shenzhen Hospital; Medical Center of Peking University; Shenzhen, Guangdong, China;4.Department of Veterinary Pathobiology; University of Missouri; Columbia, MO USA |
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| Abstract: | Nuf2 plays an important role in kinetochore-microtubule attachment and thus is involved in regulation of the spindle assembly checkpoint in mitosis. In this study, we examined the localization and function of Nuf2 during mouse oocyte meiotic maturation. Myc6-Nuf2 mRNA injection and immunofluorescent staining showed that Nuf2 localized to kinetochores from germinal vesicle breakdown to metaphase I stages, while it disappeared from the kinetochores at the anaphase I stage, but relocated to kinetochores at the MII stage. Overexpression of Nuf2 caused defective spindles, misaligned chromosomes, and activated spindle assembly checkpoint, and thus inhibited chromosome segregation and metaphase-anaphase transition in oocyte meiosis. Conversely, precocious polar body extrusion was observed in the presence of misaligned chromosomes and abnormal spindle formation in Nuf2 knock-down oocytes, causing aneuploidy. Our data suggest that Nuf2 is a critical regulator of meiotic cell cycle progression in mammalian oocytes. |
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| Keywords: | kinetochore-microtubule attachments meiosis Nuf2 oocyte spindle assembly checkpoint |
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