Transient kinetics and intermediates formed during the electron transfer reaction catalyzed by Candida albicans estrogen binding protein |
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| Authors: | Buckman J Miller S M |
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| Institution: | Department of Pharmaceutical Chemistry, University of California School of Pharmacy, San Francisco, California 94143-0446, USA. |
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| Abstract: | The transient kinetics of the reaction of the estrogen binding protein (EBP1) from Candida albicans in which hydride is transferred from NADPH to trans-2-hexenal (HXL) in two half-reactions were analyzed using UV-visible spectrophotometric and fluorometric stopped-flow techniques. The simplest model of the first half-reaction involves four steps including very rapid, tight binding (K(d)
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