Pasteur effect in the in vitro vascularly perfused rat small intestine |
| |
Authors: | J M J Lamers and W C Hü lsmann |
| |
Institution: | Department of Biochemistry I. Rotterdam Medical School, Rotterdam, The Netherlands |
| |
Abstract: | Isolated small intestine perfused in vitro with media with low oxygen concentration was found to contain low levels of ATP when compared with rat small intestine in vivo. The addition of fluorocarbon FC 75 to an erythrocyte-free perfusion medium was found to result in a high phosphate potential and a low rate of lactate production from glucose in isolated perfused small intestine, resembling the in vivo condition. This allowed the demonstration of a Pasteur effect in that replacement of oxygen by nitrogen (or the addition of 2,4-dinitrophenol) led to a rapid increase of the rate of glycolysis, and a decrease of the ATP concentration in the tissue |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |