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The binding mechanism of a peptidic cyclic serine protease inhibitor
Authors:Jiang Longguang  Svane Anna S P  Sørensen Hans Peter  Jensen Jan K  Hosseini Masood  Chen Zhuo  Weydert Caroline  Nielsen Jakob T  Christensen Anni  Yuan Cai  Jensen Knud J  Nielsen Niels Chr  Malmendal Anders  Huang Mingdong  Andreasen Peter A
Affiliation:
  • 1 Danish-Chinese Centre for Proteases and Cancer, Aarhus University, DK-8000 Aarhus C, Denmark
  • 2 Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou 350002, China
  • 3 Interdisciplinary Nanoscience Center (iNANO), Aarhus University, DK-8000 Aarhus C, Denmark
  • 4 Department of Chemistry, Aarhus University, DK-8000 Aarhus C, Denmark
  • 5 Department of Molecular Biology, Aarhus University, DK-8000 Aarhus C, Denmark
  • 6 Section for Bio-Organic Chemistry, Faculty of Life Sciences, University of Copenhagen, 1871 Frederiksberg C, Denmark
  • Abstract:
    Keywords:ITC, isothermal titration calorimetry   BSA, bovine serum albumin   RU, response units   HBS, Hepes-buffered saline   NOE, nuclear Overhauser effect   TFA, trifluoroacetic acid   uPA, urokinase-type plasminogen activator   uPAR, uPA receptor   VLK-AMC, H-  smallcaps"  >d-Val-Leu-Lys-7-amido-4-methylcoumarin   HPLC, high-pressure liquid chromatography   PEG, polyethylene glycol
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