Novel structural insights into rotavirus recognition of ganglioside glycan receptors |
| |
Authors: | Yu Xing Coulson Barbara S Fleming Fiona E Dyason Jeffrey C von Itzstein Mark Blanchard Helen |
| |
Institution: | 1 Institute for Glycomics, Gold Coast campus, Griffith University, Queensland 4222, Australia2 Department of Microbiology and Immunology, The University of Melbourne, Victoria 3010, Australia |
| |
Abstract: | Rotaviruses ubiquitously infect children under the age of 5, being responsible for more than half a million diarrhoeal deaths each year worldwide. Host cell oligosaccharides containing sialic acid(s) are critical for attachment by rotaviruses. However, to date, no detailed three-dimensional atomic model showing the exact rotavirus interactions with these glycoconjugate receptors has been reported. Here, we present the first crystallographic structures of the rotavirus carbohydrate-recognizing protein VP8? in complex with ganglioside GM3 glycans. In combination with assessment of the inhibition of rotavirus infectivity by N-acetyl and N-glycolyl forms of this ganglioside, our results reveal key details of rotavirus-ganglioside GM3 glycan recognition. In addition, they show a direct correlation between the carbohydrate specificities exhibited by VP8? from porcine and by monkey rotaviruses and the respective infectious virus particles. These novel results also indicate the potential binding interactions of rotavirus VP8? with other sialic acid-containing gangliosides. |
| |
Keywords: | STD saturation transfer difference SSRL Stanford Synchrotron Radiation Lightsource |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|