Differential Regulation of Fast Axonally Transported Proteins During the Early Response of Rat Retinal Ganglion Cells to Axotomy |
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Authors: | L Wodarczyk V K L Merrill G W Perry |
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Institution: | Neuroscience Research Laboratories, Center for Complex Systems and Department of Psychology, Florida Atlantic University, Boca Raton, Florida, U.S.A. |
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Abstract: | Abstract: We have found that the early response of axotomized rat retinal ganglion cells is characterized by the differential regulation of a number of fast axonally transported proteins. The abundance of 23 radiolabeled fast transported proteins was analyzed at 2 and 5 days after axotomy using two-dimensional gel electrophoresis. Corresponding changes in retinal GAP-43 mRNA were measured using northern analysis. Within 2 days of injury, >40% of the transported proteins analyzed, including GAP-43, showed increased labeling above control levels. Approximately 13% of transported proteins decreased below control levels, whereas the remainder did not change. Five days after axotomy, only GAP-43 and another fast transported protein, C3, continued to sustain measurable increased labeling above control levels; all previously elevated proteins appeared to have been down-regulated by this time, which corresponds to the onset of cell death. These differential changes were accompanied by parallel increases in GAP-43 mRNA. These results suggest that the molecular changes within rat retinal ganglion cells are differentially regulated within two stages subsequent to damage, initial regenerative growth followed by cell death. |
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Keywords: | Axotomy Fast axonally transported proteins GAP-43 Northern analysis Optic nerve Regeneration |
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