Influence of Sulfate-Reducing Bacteria on Outdoor Hydrogen Production by Photosynthetic Bacterium with Seawater |
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Authors: | Isamu Maeda Tadashi Mizoguchi Yoshiharu Miura Kiyohito Yagi Norio Shioji Hitoshi Miyasaka |
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Institution: | (1) Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, Osaka 565-0871, Japan , JP;(2) Mitsubishi Heavy Industries Ltd., Takasago, Hyogo 676-0008, Japan , JP;(3) Kansai Electric Power Co. Amagasaki, Hyogo 661-0974, Japan , JP;(4) RITE Nankoh Laboratories, Suminoe-ku, Osaka 559-0032, Japan , JP |
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Abstract: | The application of seawater for bacterial fermentative production is a cost-effective technology. Hydrogen production by
marine photosynthetic bacterium with seawater failed to continue after more than 10 days, and was accompanied by the formation
of hydrogen sulfide and a change in culture color from red to black. However, substrate consumption in the blackish culture
was comparable to that in a hydrogen-producing culture. A decrease in hydrogen production occurred upon the addition of sodium
sulfide at concentrations of 1.5 mM or higher. PCR analysis targeted at the 16S rDNA sequence selective for sulfate-reducing
bacteria revealed the existence of sulfate-reducing bacteria in inoculation cultures of the phototrophic bacterium and medium
for hydrogen production. Hence, the high sulfate concentration of seawater, the low oxidation-reduction potential under hydrogen-producing
conditions, and the presence of electron donors such as acetate might promote the metabolic activities of sulfate-reducing
bacteria, resulting in the deterioration of hydrogen production with seawater.
Received: 15 September 1999 / Accepted: 14 October 1999 |
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