Interpreted gene expression of human dermal fibroblasts after adipo-, chondro- and osteogenic phenotype shifts |
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Authors: | Jonathan Rakar Susanna Lönnqvist Pehr Sommar Johan Junker Gunnar Kratz |
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Institution: | 1. Experimental Plastic Surgery, Department of Clinical and Experimental Medicine, Linköping University, Sweden;2. Center for Integrative Regenerative Medicine (IGEN), Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, Sweden;3. Division of Plastic Surgery, Brigham and Women''s Hospital, Harvard Medical School, Boston, MA, USA;4. Department of Plastic Surgery, County of Östergötland, Linköping, Sweden |
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Abstract: | Autologous cell-based therapies promise important developments for reconstructive surgery. In vitro expansion as well as differentiation strategies could provide a substantial benefit to cellular therapies. Human dermal fibroblasts, considered ubiquitous connective tissue cells, can be coaxed towards different cellular fates, are readily available and may altogether be a suitable cell source for tissue engineering strategies. Global gene expression analysis was performed to investigate the changes of the fibroblast phenotype after four-week inductions toward adipocytic, osteoblastic and chondrocytic lineages. Differential gene regulation, interpreted through Gene Set Enrichment Analysis, highlight important similarities and differences of induced fibroblasts compared to control cultures of human fibroblasts, adipocytes, osteoblasts and articular chondrocytes. Fibroblasts show an inherent degree of phenotype plasticity that can be controlled to obtain cells supportive of multiple tissue types. |
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Keywords: | A-FB adipogenic media induced FB AC human adipocyte reference group C-FB chondrogenic media induced FB CC human chondrocyte reference group FB human dermal fibroblasts (control group) O-FB osteogenic media induced FB OB human osteoblast reference group |
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