| 蜻蜒拟青霉多型性的扫描电镜观察及RAPD分析 |
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| 引用本文： | 刘作易,梁宗琦,刘爱英,喻子牛.蜻蜒拟青霉多型性的扫描电镜观察及RAPD分析[J].菌物学报,2000,19(1). |
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| 作者姓名： | 刘作易 梁宗琦 刘爱英 喻子牛 |
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| 作者单位： | 1 贵州大学虫生真菌研究室； 2 华中农业大学生命科学技术学院 |
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| 基金项目： | 中科院微生物所真菌地衣开放室课题 |
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| 摘 要： | 通过电镜扫描观察，清楚地显示了靖蜒拟青霉（Ｐａｅｃｉｌｏｍｙｃｅｓ ｏｄｏｎａｔａｅ Ｌｉｕ，Ｌｉａｎｇ＆Ｌｉｕ）的两种类型产孢结构，即拟青霉型椭圆形分生抱子的链状排列和校顶孢霉型柱状分生抱子头状排列。同时将椭圆形分生孢子和柱状分生孢子进行单孢分离，对各单孢株作了培养特征观察，形态学观察和ＤＮＡ随机片段扩增分析。结果表明，在形态学上两种孢子单孢子株之间的培养特征和显微特征均无明显差异，同时具有相同的ＤＮＡ随机扩增片段电泳谱。
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| 关 键 词： | 电镜扫描 蜻蜒拟青霉 DNA随机片段多态分析 多型性 |
THE SEM OBSERVATION AND ANALYSIS OF PLEOMORPHISMOF PAECILOMYCES ODONATAE |
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| Abstract: | The SEM photographs showed clearly that Paecilomyces odonatae Liu, Liang & Liu possessed twosorts of conidiogenous structures, which were the Paecilomyces-type with ellipsoidal conidia in chain andAcremonium-type producing cylindrical conidia in a slime head. The single spore strains obtained by single sporeisolation were used in morphological observahon and RAPD analysis. The resultS showed the cultural andmorphological characteristics and the bands of DNA RAPD of the ellipsoidal single conidial strains were nomuch differences from those of cylindrical single conidial strains. |
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| Keywords: | SEM Paecilomyces odonatae RAPD Pleomorphism |
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