Application of statistical design for the optimization of microbial community of synthetic domestic wastewater |
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| Authors: | Yuancai Chen Che-Jen Lin Gavin Jones Shiyu Fu Huaiyu Zhan |
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| Institution: | (1) State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, 510640 Guangzhou, Guangdong, China;(2) Department of Civil Engineering, Lamar University, Beaumont, TX 77710-0024, USA;(3) School of Environmental Science and Engineering, South China University of Technology, 510006 Guangzhou, Guangdong, China;(4) Texas Research Institute for Environmental Studies, Sam Houston State University, Huntsville, TX 77341-2506, USA |
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| Abstract: | A fractional factorial design (FFD) and a response surface methodology (RSM) were used to optimize the inoculum composition
of six strains for treatment of synthetic domestic wastewater. The model predicted the highest overall specific substrate
utilization rate (q) of 6.88 g TOC/(d-gVSS). The value is in accordance with the actual maximum q, and is 1.5 and 1.97 times greater than those without optimization for 4 and 6 strains respectively. Additionally, the shortest
time to reach stationary phase (3.5 h) and highest maximum total organic carbon (TOC) removal efficiency (92%) were also achieved
under the optimum condition. The results indicated that the FFD and RSM are powerful screening and optimizing tools for the
microbial community. The experimental approaches enhance the overall specific rate of substrate utilization as well as other
biodegradation parameters. |
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