Chemostat optimization of biomass production of a mixed bacterial culture utilizing methanol |
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Authors: | A S Abu-Ruwaida I M Banat I Y Hamdan |
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Institution: | (1) Kuwait Institute for Scientific Research, P. O. Box 2 48 85, 13109 Safat, Kuwait |
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Abstract: | Summary A continuous culture technique was used to optimize the medium composition and growth conditions of a mixed bacterial culture utilizing methanol. The improved medium resulted in satisfactory growth, high-yield coefficients and gave a product containing reduced polysaccharide concentrations. Optimal growth and biomass yields occurred at pH 6.8 a temperature of 37° C and dissolved oxygen at >20% saturation. The maximum growth rate was 0.58 h–1 and maximum biomass yield 0.48 g g–1. The protein content of the product ranged between 81%–83%, and nucleic acid content between 10%–12%, increasing with growth rate. The amino acid profile of the mixed culture product met and, in some cases, exceeded the UN Food and Agricultural Organization standard, indicating a good source of feed protein.Offprint requests to: A. S. Abu-Ruwaida |
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