Regeneration of Picea omorika plants via organogenesis |
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| Authors: | Branka Kolevska-Pletikapić Zinka Buturović-Derić |
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| Affiliation: | (1) Department of Molecular Biology, Faculty of Science, University of Zagreb, Rooseveltov trg 6, 41000 Zagreb, Croatia;(2) Present address: Tasman Forestry Ltd., Centre for Advanced Forest Biotechnology, Te Teco, PO Box 149, Bay of Plenty, New Zealand |
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| Abstract: | Picea omorika plants were regenerated from embryo and seedling shoot tip cultures. Adventitious and axillary shoots were produced on 1/2 MS medium containing benzyladenine and kinetin. Benzyladenine was more effective in bud induction, whereas kinetin hastened shoot development. Excised shoots were elongated on 1/3 MS medium without growth regulators, multiplied with kinetin and rooted with or without indole-3-butyric acid.Abbreviations BA N6-benzyladenine - 2IP N6-( 2-isopenteny) adenine - NAA  -naphthaleneacetic acid - IAA indole-3-acetic acid - IBA indole-3-butyric acid |
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| Keywords: | embryo culture in vitro plant regeneration seedling shoot tip culture |
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