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The effect of ethanol on the lipoprotein metabolism of aortic smooth muscle cells |
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| Authors: | E Ruczkal-Pietrzak P A Wilce B C Shanley |
| Institution: | 1. Department of Biostatistics, School of Public Health, Boston University, Boston, MA, USA;2. Division of Cardiology, Department of Medicine and Cardiovascular Research Center, Massachusetts General Hospital, Boston, MA, USA;3. Harvard Medical School, Boston, MA, USA;4. Department of Epidemiology and Biostatistics, Imperial College London, London, United Kingdom;5. Department of Hygiene and Epidemiology, University of Ioannina Medical School, Ioannina, Greece;6. Framingham Heart Study, National Heart, Lung, and Blood Institute, Framingham, MA, USA;7. Population Sciences Branch, National Heart, Lung, and Blood Institute, NIH, Bethesda, MD, USA;8. Nutrition Epidemiology and Data Science, Friedman School of Nutrition Science and Policy, Tufts University, Boston, MA, USA |
| Abstract: | The effect of ethanol exposure on the binding and metabolism of bovine low density lipoprotein by bovine arterial smooth muscle cells was studied. In cells exposed to ethanol (80 mM) for 48 hr or 14 days and incubated with low density lipoprotein for 24 hr there was a reduction in the amount of low density lipoprotein internalised at all concentrations of lipoprotein. There was no effect on the rate of degradation of the low density lipoprotein and no demonstrable changes in the amount of low density lipoprotein bound to the cell surface at high concentrations of low density lipoprotein. Similar results for internalisation and degradation were obtained in a time dependent study. Binding was shown to be reduced in the ethanol treated cells (48 hr) when low concentrations (5 micrograms/ml) of low density lipoprotein were incubated for short periods (less than 3 hr). Scatchard plot analysis indicated that this reduced binding may be due to a reduction of receptor numbers in these cells. |
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