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Genetic Diversity and Characterization of a Core Collection of Malus Germplasm Using Simple Sequence Repeats (SSRs)
Authors:Sarah M Potts  Yuepeng Han  M Awais Khan  Mosbah M Kushad  A Lane Rayburn  Schuyler S Korban
Institution:1. Department of Crop Sciences, University of Illinois at Urbana?CChampaign, Urbana, IL, 61801, USA
2. Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Moshan, Wuhan, 430074, People??s Republic of China
3. Department of Natural Resources and Environmental Sciences, University of Illinois at Urbana?CChampaign, Urbana, IL, 61801, USA
Abstract:Simple sequence repeats (SSRs) were used to assess genetic diversity and study genetic relatedness in a large collection of Malus germplasm. A total of 164 accessions from the Malus core collection, maintained at the University of Illinois, were genotyped using apple SSR markers. Each of the accessions was genotyped using a single robust SSR marker from each of the 17 different linkage groups in Malus. Data were subjected to principal component analysis, and a dendrogram was constructed to establish genetic relatedness. As expected, this diverse core collection showed high allelic diversity; moreover, this allelic diversity was higher than that previously reported. Cluster analysis revealed the presence of four distinct clusters of accessions in this collection.
Keywords:
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