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Proteomic Analysis of Developing Somatic Embryos of Coffea arabica |
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| Authors: | ?ngela Tonietto Juliana Hiromi Sato Jo?o Batista Teixeira Emanuel M de Souza Fabio O Pedrosa Octávio Luiz Franco Angela Mehta |
| Institution: | 1. Centro de An??lises Prote?micas e Bioqu??micas Pos-Gradua??o em Ci??ncias Gen?micas e Biotecnologia, Universidade Catolica de Brasilia, SGAN 916, Av. W5, M??dulo C, sala 219, CEP 70790-160, Bras??lia, DF, Brazil 2. Universidade de Bras??lia, Campus Universit??rio Darcy Ribeiro, CEP 70910-900, Bras??lia, DF, Brazil 3. Embrapa Recursos Gen??ticos e Biotecnologia, Avenida W 5 Norte Final, CEP 70770-917, Bras??lia, DF, Brazil 4. Universidade Federal do Paran??, Centro Polit??cnico, Caixa Postal 19046, CEP 81531-980, Curitiba, PR, Brazil |
| Abstract: | Differential protein profiles of three stages of somatic embryogenesis, including globular, torpedo, and cotyledonary somatic embryos, of Coffea arabica cv. Catuaí Vermelho were analyzed in an attempt to better understand somatic embryogenesis in coffee plants. Somatic embryos at these different stages of development were collected from in vitro-grown cultures, and then macerated in liquid nitrogen. Proteins were extracted with phenol and further quantified using the Bradford method. The bidimensional electrophoresis analysis revealed a wide range of proteins ranging between 10 and 160?kDa and of pH values ranging from 3 to 10. Several differentially expressed proteins were identified by mass spectrometry, and some were found to be specific to these different stages of somatic embryogenesis in coffee. The enolase and 11S storage globulin proteins, for example, could be used as molecular markers for somatic embryo development stages and for embryogenic and non-embryogenic genotype differentiation, respectively. |
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