The establishment of appropriate methods for egg-activation by human PLCZ1 RNA injection into human oocyte |
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| Affiliation: | 1. Saint Mother Obstetrics and Gynecology Clinic, Institute for ART, Fukuoka 807-0825, Japan;2. Department of Virology and Parasitology, Hamamatsu University School of Medicine, Hamamatsu 431-3192, Japan;3. Department of Obstetrics and Gynecology, Juntendo University School of Medicine, Tokyo 113-0033, Japan;1. Biology Department, St. Olaf College, Northfield, MN 55057, USA;2. Department of Microbiology and Immunology, Cornell University, Ithaca, NY 14853, USA;3. College of Life Science, Fujian Normal University, Fuzhou 350108, China;4. MCD Biology, University of Colorado, Boulder, CO 80309, USA;1. Laboratory of Veterinary Embryology and Biotechnology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea;2. Department of Life Science, College of BioNano Technology, Gachon University, Incheon, Republic of Korea;3. Laboratory of Theriogenology, College of Veterinary Medicine, Kangwon National University, Chuncheon, Kangwon, Republic of Korea;1. GENERA, Centers for Reproductive Medicine, Rome, Italy;2. GENETYX, Marostica, Italy;3. The Bridge Centre, London, United Kingdom;4. School of Biosciences, University of Kent, Canterbury, United Kingdom;5. Illumina, Cambridge, United Kingdom;1. Department of Histology and Embryology, Faculty of Medicine, Institute of Health Sciences, University of Health Sciences, Istanbul 34668, Turkey;2. IVF Unit, Additional Service Building of Suleymaniye Obstetrics and Pediatrics Hospital, Istanbul Training and Research Hospital, University of Health Sciences, Istanbul 34116, Turkey |
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| Abstract: | Phospholipase C-zeta (PLCZ1), a strong candidate of egg-activating sperm factor, can induce Ca2+ oscillations and cause egg activation. For the application of PLCZ1 to clinical use, we examined the pattern of Ca2+ responses and developmental rate by comparing PLCZ1 RNA injection methods with the other current methods, such as cytosolic aspiration, electrical stimulation and ionomycin treatment in human oocytes. We found that the pattern of Ca2+ oscillations after PLCZ1 RNA injection exhibited similar characteristics to that after ICSI treatment. We also determined the optimal concentration of human PLCZ1 RNA to activate the human oocytes. Our findings suggest that human PLCZ1 RNA is a better therapeutic agent to rescue human oocytes from failed activation, leading to normal and efficient development. |
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| Keywords: | Human oocyte Egg activation PLCZ1 RNA Preimplantation development. |
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