新生婴儿源植物乳杆菌HLPL03的环境耐受性及其代谢功能低聚糖的生物学活性

【目的】解析健康新生婴儿胎便中植物乳杆菌HLPL03的益生功能，评价其环境耐受性及代谢功能低聚糖的生物学活性。【方法】通过耐受胃肠道条件、过氧化氢和抗生素试验，评估植物乳杆菌HLPL03对极端环境的耐受性；利用改良培养基，评价植物乳杆菌HLPL03代谢功能低聚糖的能力；同时，探究功能低聚糖对植物乳杆菌HLPL03抑菌活性、疏水性和黏附能力的影响。【结果】植物乳杆菌HLPL03在pH 2.5条件下培养3 h后，活菌数仍在10⁴ CFU/mL以上；在0.30%胆盐中培养6 h后，活菌数接近10⁷ CFU/mL；在1.0 mmol/L H₂O₂强氧化剂条件下培养6 h，活菌数显著升高(P<0.001)；除低聚木糖外，植物乳杆菌HLPL03能代谢多种功能低聚糖，并对常见食源性致病菌具有较强的抑制能力；棉子糖是改善植物乳杆菌HLPL03生物学活性的最佳低聚糖，其能提高菌株表面疏水性达36.1%，且促进菌株在Caco-2细胞上的黏附率由16.78%提高至42.11%。【结论】健康新生婴儿源植物乳杆菌HLPL03具有良好的抗环境胁迫能力，且其生物学活性能被棉子糖等功能低聚糖有效促进，可作为特色乳酸菌进行研究和开发。

Newborn-derived Lactiplantibacillus plantarum HLPL03: environmental tolerance and metabolism of functional oligosaccharides

Objective] To analyze the probiotic functions of Lactiplantibacillus plantarum HLPL03 derived from the stool of healthy newborns and evaluate the environmental tolerance and metabolism of functional oligosaccharides of this strain. Methods] The tolerance of L. plantarum HLPL03 to extreme conditions was evaluated by treatments with the gastrointestinal environment, hydrogen peroxide, and antibiotics. The modified media were used to evaluate the metabolism of functional oligosaccharides by L. plantarum HLPL03. Furthermore, the effects of functional oligosaccharides on the antibacterial activities, hydrophobicity, and adhesion of L. plantarum HLPL03 were investigated. Results] The viable count was above 10⁴ CFU/mL when L. plantarum HLPL03 was cultured at pH 2.5 for 3 h and close to 10⁷ CFU/mL after the strain was cultured in 0.30% bile salt for 6 h. The viable count increased significantly when L. plantarum HLPL03 was cultured with 1.0 mmol/L H₂O₂for 6 h (P<0.001). L. plantarum HLPL03 metabolized different functional oligosaccharides (except xylooligosaccharides) and inhibited common food-borne pathogens. Raffinose was the best oligosaccharide to improve the biological activity of L. plantarum HLPL03. It enhanced the surface hydrophobicity of the strain by 36.1% and increased the adhesion rate of the strain on Caco-2 cells from 16.78% to 42.11%. Conclusion] L. plantarum HLPL03 from healthy newborns has good resistance to environmental stress, with the biological activities effectively promoted by functional oligosaccharides such as raffinose, serving as characteristic lactic acid bacteria for research and development.