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Genetic strategies to analyze primary TRP channel-expressing cells in mice
Affiliation:1. Pharmacology and Toxicology, Center for Molecular Signaling (PZMS), Saarland University School of Medicine, Homburg, Germany;2. Institute of Anatomy and Cell Biology, Saarland University School of Medicine, Homburg, Germany;1. Department of Histology and Embryology, School of Medicine, University of Mostar, Bijeli Brijeg bb, 88000 Mostar, Bosnia and Herzegovina;2. Department of Anatomy, Histology and Embryology, School of Medicine, University of Split, Soltanska 2, 21000 Split, Croatia;1. Department of Anatomy, Faculty of Veterinary Science, Prince of Songkla, University, Songkhla, Thailand;2. Department of Anatomy, Graduate School of Medicine, Hokkaido University, Sapporo, Japan;3. Department of Anatomy, Graduate School of Medicine, Tohoku University, Sendai, Japan;4. Electron Microscopy Unit, Department of Anatomy, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;2. Department of Transfusion, Peking University Shenzhen Hospital, Shenzhen PKU-HKUST Medical Center, Shenzhen;3. School of Computer Science and Technology, Tianjin University, Tianjin;4. Guangdong Provincial Key Laboratory of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, China;6. State Key Laboratory of Genetic Engineering and Ministry of Education Key Laboratory of Contemporary Anthropology, School of Life Sciences, Fudan University, Shanghai;5. Stanley Ho Centre for Emerging Infectious Diseases, Li Ka Shing Institute of Medical Sciences, The Chinese University of Hong Kong, Hong Kong;11. Center for Clinical Medical Research, Department of Cancer, Shandong Provincial Zibo Central Hospital, Zibo, China;12. Institute of Molecular Medicine and Genetics, Georgia Regents University, Augusta, GA;1. Walther Straub Institute of Pharmacology and Toxicology, Faculty of Medicine, LMU Munich, Goethestr. 33, 80336 Muenchen, Germany;2. Institut für Experimentelle und Klinische Pharmakologie und Toxikologie & PZMS, Medizinische Fakultaet, Universitaet des Saarlandes, 66421 Homburg, Germany;1. Neuronal Control of Metabolism (NeuCoMe) Laboratory, Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), 08036 Barcelona, Spain;2. Program in Integrative Cell Signaling and Neurobiology of Metabolism, Department of Comparative Medicine, Yale University School of Medicine, New Haven, CT 06520, USA;3. Laboratory of Metabolism, Department of Internal Medicine Specialties, Faculty of Medicine, University of Geneva, 1211 Geneva, Switzerland;4. Institute for Research in Biomedicine (IRB Barcelona), 08028 Barcelona, Spain;5. Departament de Bioquímica i Biologia Molecular, Facultat de Biologia, Universitat de Barcelona, 08028 Barcelona, Spain;6. CIBER de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), 08036 Barcelona, Spain;7. Institute of Research in Digestive Health (IRSD) – INSERM U1220, European Associated Laboratory “NeuroMicrobiota”, University Paul Sabatier, 31024 Toulouse, France;8. Unidad de Gestión Clínica de Endocrinología y Nutrición, Instituto de Biomedicina de Sevilla (IBiS), Hospital Universitario Virgen del Rocío/Consejo Superior de Investigaciones Científicas/Universidad de Sevilla, 41013 Sevilla, Spain;9. Instituto de Investigaciones Sanitarias (IDIS), CIMUS, University of Santiago de Compostela, Santiago de Compostela 15782, Spain;10. CIBER Fisiopatología de la Obesidad y Nutrición (CIBERobn), 15706 Santiago de Compostela, Spain;11. Diabetes and Obesity Research Laboratory, Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), 08036 Barcelona, Spain;12. Department of Physiological Sciences, University of Barcelona, 08907 Barcelona, Spain;13. Institut d’Investigació Biomèdica de Bellvitge (IDIBELL), L’Hospitalet de Llobregat, 08908 Barcelona, Spain;14. Department of Anatomy and Hystology, University of Veterinary Medicine, Budapest 1078, Hungary;15. Department of Endocrinology and Nutrition, Hospital Clínic. School of Medicine, University of Barcelona, 08036 Barcelona, Spain
Abstract:Transient receptor potential (TRP) ion channels regulate fundamental biological processes throughout the body. TRP channel dysfunction has been causally linked to a number of disease states and thus establishes these channels as promising therapeutic targets. In order to dissect the physiological role of individual TRP channels in specific tissues, a detailed understanding of the expression pattern of the different TRP channels throughout the organism is essential. We provide an overview of recent efforts to generate novel TRP channel reporter mouse strains for all 28 TRP channels encoded in the mouse genome to understand expression of these channels with a single-cell resolution in an organism-wide manner. The reporter mice will enable both the visualization and manipulation of all primary TRP channel-expressing cells allowing an unprecedented wealth in variety to investigate TRP channel function in vivo. As proof of principle, we provide preliminary results documenting TRPM5 expression throughout the entire body of juvenile and adult mice.
Keywords:TRP channels  TRPM5  Gene targeting  Homologous recombination  Knock-in  Genetic labeling  Internal ribosomal entry site  Cre recombinase  ROSA26  τGFP
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