Exploring the Ca2+-dependent synaptic dynamics in vibro-dissociated cells |
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| Affiliation: | 1. Department of Biochemistry and Molecular Biology, The University of Texas Medical Branch at Galveston, 301 University Boulevard, Galveston, TX 77555-1053, USA;2. Department of Obstetrics and Gynecology, The University of Texas Medical Branch at Galveston, 301 University Boulevard, Galveston, TX 77555-1053, USA;3. The Sealy Center for Structural Biology, Sealy Center for Cancer Cell Biology, The University of Texas Medical Branch at Galveston, 301 University Boulevard, Galveston, TX 77555-1053, USA;1. Department of Psychology, Florida State University, Tallahassee, FL, USA;2. Florida State University, Tallahassee, FL, USA |
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| Abstract: | Dynamic alteration of the synaptic strength is one of the most important processes occurring in the nervous system. Combination of electrophysiology, confocal imaging and molecular biology led to significant advances in this research field. Yet, a progress in this area, in particular in studies of changes in the quantal behavior of central synapses and impact of glial cells on individual synapses, is hampered by technical difficulties of resolving small quantal synaptic currents. In this paper we will show how the technique of non-enzymatic vibro-dissociation, which enables to isolate living neurons avoiding artifacts of cell culture and preserving functional synapse, can be used to obtain a valuable information on fine details and mechanisms of synaptic plasticity. In particular, we will describe our recent results on Ca2+-dependent modulation of the postsynaptic AMPA and NMDA receptors in the individual synaptic boutons. |
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| Keywords: | Synaptic strength Receptor trafficking Quantal synaptic current Astrocyte Vesicular release ATP receptor NMDA receptor Single-synapse response |
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