Purification of Toxoplasma gondii oocysts by cesium chloride gradient |
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Authors: | Dumètre Aurélien Dardé Marie-Laure |
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Affiliation: | UPRES EA 3174, Neuroparasitologie et neuroépidémiologie tropicale, Faculté de Médecine, 2 Rue du Dr Marcland, F-87025 Limoges, France. |
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Abstract: | We describe the use of a cesium chloride (CsCl) gradient as an improvement for the purification of Toxoplasma gondii oocysts from concentrated suspensions. After concentration by sucrose flotation, this technique gives a > 96% recovery of very pure unsporulated or sporulated oocysts, but requires "fresh" oocysts (< or = 10 weeks of age). This material is suitable for biochemical and immunological analyses of environmentally resistant T. gondii oocysts. |
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