Plasma Sex Steroid Levels and Steroidogenesis in the Gonad of the Self-fertilizing Fish Rivulus marmoratus |
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Authors: | Masako Minamimoto Yoshitaka Sakakura Kiyoshi Soyano Yoshiaki Akaba Atsushi Hagiwara |
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Institution: | (1) Graduate School of Science and Technology, Nagasaki University, Bunkyo 1-14, 852-8521 Nagasaki, Japan;(2) Faculty of Fisheries, Nagasaki University, Bunkyo 1-14, 852-8521 Nagasaki, Japan;(3) Institute for East China Sea Research, Nagasaki University, Taira 1551-7, 851-2213 Nagasaki, Japan |
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Abstract: | Synopsis The mangrove killifish, Rivulus marmoratus, is the only known self-fertilizing vertebrate. This species is sexually dimorphic; sexually mature individuals are either
hermaphrodite or primary and secondary males. Although the mangrove killifish has a unique reproductive strategy, there has
been no study on the reproductive endocrinology of this species. Thus we investigated plasma sex steroid hormone levels and
steroidogenesis in the gonads of R. marmoratus by enzyme linked immunosorbent assay (ELISA). Plasma 17β-estradiol (E2) and 11-ketotestosterone (11-KT) were detected both
in hermaphrodite and in primary male. Ovarian follicles (follicle-enclosed oocytes) from hermaphrodites, which were categorized
into early yolk stage and late yolk stage, and testis tissue of primary males were cultured with different concentrations
of 17α-hydroxyprogesterone (OHP) or testosterone (T) for 24 h. Production of T, E2, 11-KT and 17α-20 β-dihydroxy-4-pregnen-3-one
(17α,20β-P) in the medium from tissue culture were measured by ELISA. Early and late ovarian follicles of hermaphrodites and
testis pieces of primary males synchronously secreted E2, 11-KT, and 17α,20β-P following incubation with OHP or T. We conclude
that both hermaphrodite and primary male of the mangrove killifish secrete estrogen, androgen, and progestin synchronously. |
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Keywords: | mangrove killifish steroid hormone hermaphrodite tissue culture |
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