Prevalence and distribution of introns in non-ribosomal protein genes of yeast |
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Authors: | Jose R Rodriguez-Medina and Brian C Rymond |
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Institution: | (1) Department of Biochemistry, University of Puerto Rico, Medical Sciences Campus, P.O. Box 365067, 00936-5067 San Juan, Puerto Rico, USA;(2) T. H. Morgan School of Biological Sciences, Molecular and Cell Biology Group, University of Kentucky, 101 Morgan Building, 40506-0225 Lexington, Kentucky, USA |
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Abstract: | Relatively few genes in the yeast Saccharornyces cerevisiae are known to contain intervening sequences. As a group, yeast ribosomal protein genes exhibit a higher prevalence of introns when compared to non-ribosomal protein genes. In an effort to quantify this bias we have estimated the prevalence of intron sequences among non-ribosomal protein genes by assessing the number of prp2-sensitive mRNAs in an in vitro translation assay. These results, combined with an updated survey of the GenBank DNA database, support an estimate of 2.5% for intron-containing non-ribosomal protein genes. Furthermore, our observations reveal an intriguing distinction between the distributions of ribosomal protein and non-ribosomal protein intron lengths, suggestive of distinct, gene class-specific evolutionary pressures. |
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Keywords: | Yeast prp2 Intron Genome |
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