响应面法优化微波提取绞股蓝愈伤组织中人参皂苷Rb1的工艺研究

以绞股蓝愈伤组织为原料,优化绞股蓝人参皂苷Rb1的微波提取工艺,在单因素试验的基础上,选择液料比、微波功率和微波处理时间为自变量,人参皂苷Rb1为响应值,采用响应曲面法设计、分析研究各自变量及其交互作用对人参皂苷Rb1提取率的影响.利用响应面分析方法,模拟得到二次多项式回归方程的预测模型,并确定人参皂苷Rb1微波辅助提取工艺的最佳条件为：料液比1：20（g/mL）,处理时间6 min,微波功率200 W.在此最佳工艺条件下,人参皂苷Rb1得率为3.95 mg/g.

Optimization of Microwave-assisted Extraction of Ginsenoside Rb1from Gynostemma pentaphyllum Callus Using Response Surface Analysis

Microwave was applied to the extraction of gypenoside Rb1 from Gynostemma pentaphyllum Callus, single factor experiments involving three technological parameters including material/liquid ratio, microwave power and extrac- tion duration were done for following investigations on the interactive effects of the parameters on ginsenoside Rb1 using central composite design（CCD） combined with response surface methodology. In the optimization, a quadratic polyno- mial regression model was developed for ginsenoside Rb1 yield as a response to the above parameters. Results showed that the optimal values of technological parameters for using deionized water to extract POP were as follows ： material/liq- uid ratio 1 ： 20 g/mL, extraction duration 6 rain, microwave power 200 W. Under these conditions, the yield of gypeno- side Rb1was 3.95 mg/g.