A novel approach to nonradioactive hybridization assay of nucleic acids using stained latex particles. |
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Authors: | T I Vener,M F Turchinsky,V D Knorre,S N Lukin YuV Y" >Shcherbo,V P Zubov,E D Sverdlov |
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Affiliation: | Shemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences, Moscow. |
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Abstract: | The paper describes a sensitive latex hybridization assay (LHA) method applied for indirect detection of biotinylated nucleic acid hybrids immobilized on a synthetic membrane. The biotinylated hybrids were visualized by means of latex particles containing the fluorescent dye pyronine G and coated with streptavidin; 1.6 and 0.3 pg of lambda-phage DNA was detected by dot blot hybridizations on nylon membrane and polyethyleneimine-cellophane, respectively. The assay sensitivity was increased by three orders of magnitude over that with fluorescently labeled probes due to encapsulation of the fluorescent dye in polymer particles. LHA is simple (single-stage detection procedure), fast, and more sensitive than any of the other nonradioactive hybridization methods. |
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