1,10-Phenanthroline-cuprous ion complex, a potent inhibitor of DNA and RNA polymerases. |
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Authors: | V D'Aurora A M Stern D S Sigman |
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Institution: | Department of Biological Chemistry, School of Medicine and Molecular Biology Institute University of California Los Angeles, California 90024 USA |
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Abstract: | The inhibition by 1,10-phenanthroline of DNA polymerase I has recently been attributed to the formation in the assay mixtures of a unique and effective inhibitor, the 2:1 1,10-phenanthroline-cuprous ion complex (1). We have now found that this coordination complex is also an effective inhibitor of DNA dependent RNA polymerase, Micrococcus luteus DNA dependent DNA polymerase, and T-4 DNA dependent DNA polymerase. This conclusion is based either on the requirement of a thiol for 1,10-phenanthroline inhibition or on the reversal of 1,10-phenanthroline inhibition by the non-inhibitory cuprous ion specific chelating agent 2,9-dimethyl-1,10-phenanthroline. 2,2′,2″-Terpyridine is also very effective at relieving 1,10-phenanthroline inhibition. The reversal of 1,10-phenanthroline inhibition should be attempted before it is claimed that 1,10-phenanthroline inhibits any polymerases by coordinating a zinc ion at the active site. |
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