Expression of bioactive human interferon-gamma in transgenic rice cell suspension cultures |
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Authors: | Tzy-Li Chen Yi-Ling Lin Yi-Ling Lee Ning-Sun Yang Ming-Tsair Chan |
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Affiliation: | (1) Academia Sinica, Institute of BioAgricultural Sciences, Taipei, 115, Republic of China;(2) Academia Sinica, Institute of BioAgricultural Sciences, Taipei, 115, Republic of China |
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Abstract: | We investigated the possibility of producing the therapeutic recombinant cytokine, Interferon-gamma (IFN- ), in transgenic rice cell (Oryza sativa, cultivar TNG67) suspension cultures. We tested expression of two vector constructs, each harboring an Amy3 leader peptide and a C-terminus His 6 tag fused to a human IFN- cDNA, one driven by a sucrose-starvation inducible promoter (rice Amy3 promoter) and the other by a constitutive maize ubiquitin promoter, in rice cell suspensions, introduced via Agrobacterium tumefaciens. There was a significant difference in the amounts of recombinant IFN- protein produced by the Ups and Amy cell lines, as cytosolic and secretory proteins respectively. Immunological analysis of IFN- recombinant protein conferred a dose-dependent anti-dengue virus activity in human A549 cells, similar to the commercial product. We discuss the attractive attributes of using rice cell suspension system for the expression of therapeutic recombinant IFN- . |
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Keywords: | antiviral activity recombinant human interferon-gamma transgenic rice suspension cells |
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