Selective fluorescence detection of 8-oxoguanosine with 8-oxoG-clamp |
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Authors: | Nakagawa Osamu Ono Sayaka Tsujimoto Akira Li Zhichun Sasaki Shigeki |
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Affiliation: | Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan. |
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Abstract: | 8-oxoguanosine, which is derived from the oxidation of guanosine (dG), is known to induce transversion mutations (G:C-->T:A) in DNA. The compounds with a small molecular weight for recognizing 8-oxoG were designed on the basis of the structure of the G-clamp, which is reported to have selective affinity toward guanosine. The G-clamp derivatives with the additional binding units toward 8-oxoG were effectively synthesized and named "8-oxoG-clamps." The 8-oxoG-clamp completely discriminated 8-oxoG from other nucleosides by fluorescence quenching. |
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