| 1株产细菌纤维素芽胞杆菌的分离及鉴定 |
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| 引用本文： | 朱宏阳,姚俊,冯珊,李泳宁,林伟铃.1株产细菌纤维素芽胞杆菌的分离及鉴定[J].生物加工过程,2014(5):80-84. |
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| 作者姓名： | 朱宏阳 姚俊 冯珊 李泳宁 林伟铃 |
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| 作者单位： | 福建卫生职业技术学院药学系;南京医科大学基础医学院; |
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| 基金项目： | 福建省自然科学青年基金(2012J05015);福建省教育厅A类科技项目(JA12430) |
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| 摘 要： | 从残次水果中分离出1株菌株ZF-7,其产物经纤维素特异性染色反应、红外光谱分析及纤维素酶水解实验后,被确定为细菌纤维素。在对ZF-7菌株常规形态学及生理生化特性鉴定的基础上,对部分长度的16S rDNA同源性进行了分析,发现ZF-7菌株与解淀粉芽胞杆菌相似度可达99.5%,现命名为Bacillus amyloliquefaciens ZF-7。对ZF-7菌株在振荡培养和静置培养条件下的发酵性能进行了初步考察,得到细菌纤维素产率分别为6.6和6.2 g/L。
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| 关 键 词： | 细菌纤维素 解淀粉芽胞杆菌 筛选 鉴定 |
Isolation and identification of bacterial cellulose producing strain |
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| Institution: | ZHU Hongyang,YAO Jun,FENG Shan,LI Yongning,LIN Weiling ( 1. Department of Pharmacy, Fujian Health College, Fuzhou, 350101, China; 2. School of Basic Medical Science, Nanjing Medical University, Nanjing 210029, China) |
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| Abstract: | Strain ZF-7 was isolated from bad fruits. Its product was determined as bacterial cellulose（BC） in view of the results of cellulose special reaction,FT-IR spectroscopy analysis and cellulose hydrolysis experiments. Based on morphology,physiological and biochemical characteristics,ZF-7 was identified by 16 S rDNA sequences and systematic analysis. The results indicated that 16 S rDNA sequences of ZF-7 had 99. 5% similarity to the partial sequence of Bacillus amyloliquefaciens,suggesting that ZF-7 was categorized as a subspecies of B. amyloliquefaciens, and denominated as B. amyloliquefaciens ZF-7. The fermentation performance of ZF-7 under shaking and static flasks was inspected preliminarily,and the BC production was 6. 6 and 6. 2 g /L,respectively. |
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| Keywords: | bacterial cellulose Bacillus amyloliquefaciens isolation identification |
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