Contributions of cell wall and metal-binding peptide to Cd- and Cu-tolerances in suspension-cultured cells of tomato |
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Authors: | Masahiro Inouhe Madoka Mitsumune Hiroshi Tohoyama Masanori Joho Tetsuo Murayama |
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Institution: | (1) Department of Biology, Faculty of Science, Ehime University, 790 Matsuyama, Japan |
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Abstract: | Possible roles of cell wall and cytoplasmic peptides in the tolerance of cells to Cu2+ and Cd2+ ions were studied in suspension-cultured cells of tomato (Lycopersicon esculentum L. cv. Palace). Cu2+ and Cd2+ ions inhibited growth of wild type cells at concentrations more than 100 and 200 μM, respectively. Tomato cells readily developed
tolerance to Cd2+ ions up to 1 mM but not to Cu2+ ions, after repeated subculturings in the presence of the respective ions. Such a metal-specific adaptation of cells was
not due to the difference in the total uptakes between Cd2+ and Cu2+ ions by cells. Wild-type cells accumulated Cd2+ preferentially into the cytoplasmic peptide fraction and Cu2+ into the cell-wall fraction, when grown under the subtoxic metal conditions. Under excess metal conditions, Cd-tolerant cells
produced greater amounts of Cd-binding peptides in the cytoplasm and retained lesser amounts of Cd2+ ions in the cell wall than did wild-type cells. In contrast, tomato cells grown in the presence of Cu2+ ions synthesized no detectable amounts of Cu-binding peptides in the cytoplasm and retained most of the Cu2+ in the cell-wall fraction, irrespective of cell lines.
These results suggested that the cytoplasmic peptides rather than cell wall properties have a primary role in the response
of tomato cells to excess metal environments. |
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Keywords: | Cd- and Cu-tolerance cell wall tomato (Lycopersicon esculentum L ) phytochelatin [poly (γ -glutamylcysteinyl)-glycine] |
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