Improved indirubin production in a two-phase suspension culture of Polygonum tinctorium using dimethylpolysiloxane |
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| Authors: | GW Bae CS Chung KI Kim CH Park HJ Lee YA Chae IS Chung |
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| Institution: | (1) Department of Genetic Engineering, Kyung Hee University, Suwon, Korea;(2) Department of Chemical Engineering, Kyung Hee University, Suwon, Korea;(3) RCNBMA, Seoul National University, Suwon, Korea |
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| Abstract: | Dimethylpolysiloxane (DMPS), used as a second phase in a suspension culture of Polygonum tinctorium, increased indirubin production by up to 259% and 16% in shake flask and air-lift bioreactors, respectively. In the shake flask culture, indirubin production increased by up to 248 mg/l using 90% v/v DMPS (volume of DMPS/volume of medium). In an air-lift bioreactor culture grown at a perfusion rate of 0.05 day–1 the indirubin concentration reached a maximum of 130 mg/l using 50% (v/v) DMPS. For the same DMPS content, the lower indirubin production in an air-lift bioreactor was probably due to the mass transfer limitation of indirubin between the two phases. © Rapid Science Ltd. 1998 |
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| Keywords: | indirubin two-phase suspension culture Polygonum tinctorium dimethylpolysiloxane |
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