Biochemical differences between embryogenic and nonembryogenic callus of Picea abies (L.) Karst

Both embryogenic and nonembryogenic calli of Picea abies (L.) Karst. were initiated from the hypocotyl region of immature embryos. The two callus phenotypes were manually separated and subsequently maintained independently, but under identical culture conditions. Biochemical analysis of the two phenotypes revealed significant differences in ethylene evolution rate and in concentrations of glutathione and total reductants. Due to the constancy of the genetic background, age and growth conditions of the two callus types, differences in the measured quantities are not likely to be traceable to the genetic origin of the callus and serve to highlight biochemical changes associated with somatic embryogenesis in Norway spruce.Abbreviations GSH glutathione - 2,4-D 2,4-dichlorophenoxyacetic acid - BA N⁶-benzyl adenine - E embryogenic - NE nonembryogenic - NS Norway spruce