Attached and free-living bacteria: Production and polymer hydrolysis during a diatom bloom |
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| Authors: | M. Middelboe M. Søndergaard Y. Letarte N. H. Borch |
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| Affiliation: | (1) Freshwater Biological Laboratory, University of Copenhagen, 51 Helsingørsgade, DK-3400 Hillerød, Denmark;(2) Roskilde University Library, P.O. Box 258, DK-4000 Roskilde, Denmark;(3) Present address: The Royal Veterinary and Agricultural University, Department of Ecology and Molecular Biology, Microbiology Section, Rolighedsvej 21, DK-1958 Frederiksberg C, Copenhagen, Denmark;(4) Present address: College of Marine Studies, University of Delaware, 19958 Lewes, Delaware, USA |
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| Abstract: | Abundance, production and extracellular enzymatic activity of free-living and attached bacteria were measured during the development and collapse of a spring bloom in a eutrophic lake. Free-living bacteria accounted for most of the total bacterial production during the first part of the bloom. Their production had a significant positive correlation to chlorophyll (P < .01) and polysaccharide concentration (P < .02) and to potential  -glucosidase and aminopeptidase activity (P < .05), suggesting that algal release of dissolved polymeric compounds provided an important carbon source for bacterial production. As the bloom collapsed, we observed a change in the activity and structure of the microbial community. The mean contribution of attached bacteria to total bacterial production increased from 12% during the first part of the bloom to 26% at the end. Also, the extracellular enzymatic activity of attached bacteria increased as the bloom collapsed and constituted up to 75% of the total hydrolytic activity. An estimated disparity between hydrolytic activity and the corresponding carbon demand of attached bacteria suggested a net release of dissolved organic compounds from organic particles via polymer hydrolysis by attached bacteria.Correspondence to: M. Middelboe |
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