Production of seedlings from ovules excised at the zygote stage in Lilium spp. |
| |
Authors: | Ikeda Noriko Niimi Yoshiji Han Dong-Sheng |
| |
Affiliation: | (1) Faculty of Agriculture, Niigata University, 2-8050 Ikarashi, Niigata, 950-2181, Japan |
| |
Abstract: | The present study was undertaken to establish a culture system for ovules excised at the zygote stage in Lilium spp. Ovules of Lilium × `Connecticut King' and L. × `Enchantment' were excised together with placental tissue 3, 5, and 10 days after pollination (DAP) and cultured on B5 medium and half-strength B5 medium containing sucrose at different concentrations. In vitro embryo development in ovules cultured at 3 DAP was influenced by the basal media and the sucrose concentration. The half-strength B5 medium with 9% sucrose was the best condition, but only a few ovules isolated from placental tissue developed into seedlings. Application of embryo culture, in which embryos were excised from ovules after 14 weeks of ovule-with-plancetal-tissue culture, greatly improved the production of seedlings. The present study indicates that a two-step culture procedure, ovule-with-placental-tissue culture and embryo culture, make it possible to produce seedlings from ovules just after fertilization. |
| |
Keywords: | in vitro embryo development lily sucrose concentration |
本文献已被 SpringerLink 等数据库收录! |
|