用蛋白印迹确认实验检查"人类免疫缺陷病毒抗体不确定"结果的分析

目的 探讨蛋白印迹实验(WB)“人类免疫缺陷病毒(HIV)抗体不确定”结果的特点、产生的原因、WB确认实验存在的问题及可能的改善措施。方法 归纳分析本实验室2004～2005年的WB检测结果为“HIV抗体不确定”者的人群分布特点、实验检测特点、受检者随访复检及抗体转归情况。结果 “HIV抗体不确定”者人员构成中相对健康的自愿咨询检测者、献血员以及孕产妇占50%,“HIV抗体不确定”者随访困难、复检率较低;WB实验存在假阳性、尤以P24带较为严重。结论 “HIV抗体不确定”结果与WB实验的假阳性有关,实验室应采取应对措施尽可能减少“HIV抗体不确定”及对结果进行准确解释。

Analysis of indeterminate HIV Western blot profiles

Objective To explore the characteristic and reason for indeterminate HIV Western Blot(WB),the limitation of WB and the possible amendatory measures.Methods Summing up and analysis the distribution of the indeterminate HIV WB among the tested people,the laboratory test results,the follow-up and the final HIV antibody outcome.Results The relative healthy people including the people of voluntary counseling and testing,the blood donors and pregnant women make up 50% of the indeterminate HIV WB,the follow-up for the indeterminate HIV WB is difficult and the there are few people of the indeterminate HIV WB have HIV antibody test again.There is false-positive in WB test,especially the false-positive of P24 is serious.Conclusion The indeterminate HIV WB is related to the false positive of WB test,measures should be taken to reduce the indeterminate HIV WB and to interpret the result accurately.