| 流体力学注射IL-35质粒对ConA诱导小鼠肝损伤的保护作用 |
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| 引用本文： | 周艳春,李明才,牛青霞,柳晓金,谢燕飞. 流体力学注射IL-35质粒对ConA诱导小鼠肝损伤的保护作用[J]. 现代生物医学进展, 2010, 10(6) |
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| 作者姓名： | 周艳春 李明才 牛青霞 柳晓金 谢燕飞 |
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| 作者单位： | 汕头大学医学院炎症与免疫性疾病研究所,广东,汕头,514041 |
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| 摘 要： | 目的:观察流体力学注射白细胞介素35(IL-35)质粒对刀豆蛋白A(ConA)诱导的小鼠肝损伤的干预效果。方法:通过流体力学注射将pcDNA3.1-IL-35导入C57BL/6小鼠体内,对照组注射pcDNA3.1。24h后,以ConA(15mg/kg)尾静脉注射建立小鼠肝损伤模型,检测血清ALT活性和肝组织病理变化。结果:流体力学注射pcDNA3.1-IL-3524h后,肝组织有IL-35mRNA和蛋白的表达。与对照组相比,pcDNA3.1-IL-35预处理组小鼠血清ALT水平明显降低,肝组织病理损伤明显减轻。结论:流体力学注射IL-35质粒对ConA诱导的小鼠肝损伤具有保护作用。
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| 关 键 词： | 白细胞介素35 刀豆蛋白A 肝损伤 流体力学注射 |
Protective Effect of Hydrodynamic-Based Delivery of Interleukin-35 Gene on Liver Injury Induced by Concanavalin A in Mice |
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| ZHOU Yan-chun,LI Ming-cai,NIU Qing-xia,Liu xiao-jin,XIE Yan-fei. Protective Effect of Hydrodynamic-Based Delivery of Interleukin-35 Gene on Liver Injury Induced by Concanavalin A in Mice[J]. Progress in Modern Biomedicine, 2010, 10(6) |
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| Authors: | ZHOU Yan-chun LI Ming-cai NIU Qing-xia Liu xiao-jin XIE Yan-fei |
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| Affiliation: | ZHOU Yan-chun,LI Ming-cai,NIU Qing-xia,Liu xiao-jin,XIE Yan-fei (Institute of Inflammation , Immune Diseases,Medical College,Shantou University,515041,Shantou,China) |
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| Abstract: | Objective: To study the effect of hydrodynamics-based delivery of IL-35 gene on liver injury induced by Concanavalin A in mice.Methods: The murine model was established by intravenous injection of 15 mg/ kg Con A,IL-35-treated mice were injected with pcDNA3.1-IL-35 and control mice with pcDNA3.1 using hydrodynamics-based gene delivery 24 hours prior con A injection.The degree of liver injury was assessed by serum transaminase analysis and hepatic histopathology.Results: The expression of IL-35 mRNA and prot... |
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| Keywords: | Interleukin-35 Con A Liver injury Hydrodynamic-based gene delivery |
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