Establishment of a novel ovine kidney cell line for isolation and propagation of viruses infecting domestic cloven-hoofed animal species |
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| Authors: | Katsuyoshi Matsuura Yasuo Inoshima Ken-ichiro Kameyama Kenji Murakami |
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| Institution: | (1) National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba Ibaraki, 305-0856, Japan;(2) Department of Veterinary Medicine, Gifu University, 1-1 Yanagido, Gifu Gifu, 501-1193, Japan; |
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| Abstract: | A sheep kidney-derived cell line, FLK-N3, was successfully established after serial (>100) passages. Persistent infection
of this cell line with viruses and mycoplasma was not detected. The cells grew well and showed susceptibility to a wide variety
of viruses derived from ovine, bovine, and porcine species, including orf virus, maedi visna virus, bovine herpesvirus 1,
bovine parainfluenza virus 3, bovine viral diarrhea viruses 1 and 2, bovine coronavirus, bovine respiratory syncytial virus,
bovine enterovirus, suid herpesvirus 1, and porcine enterovirus. These results suggest that the FLK-N3 cell line could be
useful for isolation and propagation of viruses that affect cloven-hoofed animals. |
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