The action of cobra venom phospholipase A2 isoenzymes towards intact human erythrocytes |
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Authors: | B. Roelofsen M. Sibenius Trip H.M. Verheij J.L. Zevenbergen |
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Affiliation: | Laboratory of Biochemistry, State University of Utrecht, Transitorium III, Padualaan 8, De Uithof, 3584 CH Utrecht The Netherlands |
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Abstract: | 1. 1. Cobra venom phospholipase A2 from three different sources has been fractionated into different isoenzymes by DEAE ion-exchange chromatography. 2. 2. Treatment of intact human erythrocytes with the various isoenzymes revealed significant differences in the degree of phosphatidylcholine hydrolysis in those cells. 3. 3. It is argued that the plateaus observed in dose-response curves for such treatments may be caused by an increase in lateral surface pressure within the outer half of the membrane due to the production of free fatty acids and lyso-compounds. Keywords: Phospholipase A2 isoenzyme; Snake venom; Phosphatidylcholine hydrolysis; Fatty acid; (Naja naja,Erythrocyte) |
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Keywords: | Phospholipase A2 isoenzyme Snake venom Phosphatidylcholine hydrolysis Fatty acid (Naja naja,Erythrocyte) |
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