BALB/c和ICR小鼠的学习记忆能力等行为学研究

目的研究不同品系小鼠学习记忆能力的差异,为学习记忆的基础研究提供应用信息。方法80只BALB/c和80只ICR小鼠分别分为Morris水迷宫组、跳台组、穿梭组、ROTA-ROD组,每组20例,进行学习记忆能力及行动能力测试。结果水迷宫组在9轮水迷宫训练学习期BALB/c小鼠空间学习记忆能力没有明显提高。ICR小鼠从9轮水迷宫训练学习期的第4次开始,逃避潜伏期显著缩短,与前3次相比差异有显著性(P<0.001)。跳台组ICR和BALB/c小鼠训练前后5 min内错误次数及跳下潜伏期差异均具有显著性。穿梭组ICR小鼠学习期与记忆期主动逃避次数、被动逃避次数及电击时间的差异均有显著性,而BALB/c小鼠训练前后主动逃避次数、被动逃避次数及电击时间的差异均无显著性。ROTA-ROD组ICR小鼠的跑步动作维持时间显著高于BALB/c小鼠,其差异有显著性。结论以上结果提示在进行某些学习记忆实验时,使用ICR小鼠优于BALB/c小鼠。

Behavioral Study on Learning and Memory Ability of BALB/c and ICR Mice

Objective In order to provide valuable information for learning and memory basic study,a series of behavioral experiments were carried out to explore the difference of learning and memory ability of different strain mice.Method 80 BALB/c and 80 ICR mice were randomly divided into Morris water maze test group, jumping estrade test group,shuttle box test group and rota-rod test group.The learning and memory ability of the mice were assessed.Results Morris water maze test: Escape times of BALB/c mice in Morris maze had no significant change in their space learning and memory ability during the 9 runs water maze training.However,escape time of ICR mice was gradually decreased.From the 4~th run to the 9~th run,the escape time of ICR mice was significantly decreased in comparison with the preceding 3 runs(P<0.001).Jumping estrade test: wrong times in 5 minutes and jumping latent time of ICR mice before and after jumping estrade experiment had significant differences,respectively.Shuttle box test: The positive escape times,passive escape times,electro-stimulus time of ICR mice pro-shuttle box test training were higher than those of post-shuttle box test training,respectively.But those of BALB/c mice had no significant difference between pro-shuttle and post-shuttle box test training.ROTA-ROD Test: the keep-running time of ICR mice in ROTA-ROD test was significantly higher than that of BALB/c mice.Conclusion These results suggest that ICR mice are more suitable for some kind of learning and memory experiments than BALB/c mice.