Expression and intracellular localization of TBC1D9, a Rab
GTPase-accelerating protein,in mouse testes |
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| Authors: | Yutaka Nakamura Atsushi Asano Yoshinao Hosaka Takashi Takeuchi Toshihiko Iwanaga Yoshiaki Yamano |
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| Affiliation: | 1)Laboratory of Veterinary Biochemistry, Faculty of Agriculture, Tottori University, Japan;2)Laboratory of Veterinary Anatomy, Faculty of Agriculture, Tottori University, Japan;3)Laboratory of Laboratory Animal Science, Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan;4)Laboratory of Histology and Cytology, Department of Functional Morphology, Graduate school of Medicine, Hokkaido University, Sapporo, Hokkaido 060-8638, Japan |
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| Abstract: | Membrane trafficking in male germ cells contributes to their developmentvia cell morphological changes and acrosome formation. TBC familyproteins work as Rab GTPase accelerating proteins (GAPs), which negatively regulate Rabproteins, to mediate membrane trafficking. In this study, we analyzed the expression of aRab GAP, TBC1D9, in mouse organs and the intracellular localization of the gene products.Tbc1d9 showed abundant expression in adult mice testis. We found thatthe Tbc1d9 mRNA was expressed in primary and secondary spermatocytes, andthat the TBC1D9 protein was expressed in spermatocytes and round spermatids. In 293Tcells, TBC1D9-GFP proteins were localized in the endosome and Golgi apparatus.Compartments that were positive for the constitutive active mutants of Rab7 and Rab9 werealso positive for TBC1D9 isoform 1. In addition, TBC1D9 proteins were associated with Rab7and Rab9, respectively. These results indicate that TBC1D9 is expressed mainly inspermatocytes, and suggest that TBC1D9 regulates membrane trafficking pathways related toRab9- or Rab7-positive vesicles. |
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| Keywords: | Rab7 Rab9 Spermatogenesis TBC1D9 |
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