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A pseudovirus system enables deep mutational scanning of the full SARS-CoV-2 spike
Affiliation:1. Basic Sciences Division and Computational Biology Program, Fred Hutchinson Cancer Center, Seattle, WA 98109, USA;2. Department of Genome Sciences & Medical Scientist Training Program, University of Washington, Seattle, WA 98109, USA;3. Molecular and Cellular Biology Graduate Program, University of Washington, Seattle, WA 98109, USA;4. Department of Immunology and Microbiology, The Scripps Research Institute, La Jolla, CA 92037, USA;5. IAVI Neutralizing Antibody Center, The Scripps Research Institute, La Jolla, CA 92037, USA;6. Consortium for HIV/AIDS Vaccine Development (CHAVD), The Scripps Research Institute, La Jolla, CA 92037, USA;7. Ragon Institute of Massachusetts General Hospital, MIT & Harvard, Cambridge, MA 02139, USA;8. Aaron Diamond AIDS Research Center, Columbia University Vagelos College of Physicians and Surgeons, New York, NY, USA;9. Department of Microbiology and Immunology, Columbia University Vagelos College of Physicians and Surgeons, New York, NY 10032, USA;10. Division of Infectious Diseases, Department of Medicine, Columbia University Vagelos College of Physicians and Surgeons, New York, NY 10032, USA;11. University of Washington, Department of Medicine, Division of Allergy and Infectious Diseases, Seattle, WA, USA;12. Biozentrum, University of Basel, Basel, Switzerland;13. Swiss Institute of Bioinformatics, Lausanne, Switzerland;14. Howard Hughes Medical Institute, Seattle, WA 98195, USA
Abstract:
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  • Keywords:SARS-CoV-2  deep mutational scanning  antibody neutralization  pseudovirus  antibody escape  spike  Omicron  Delta  BA.1
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