Effects of quorum sensing autoinducer degradation gene on virulence and biofilm formation of <Emphasis Type="Italic">Pseudomonas aeruginosa</Emphasis> |
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Authors: | Wang Yao Dai Yue Zhang Yong Hu YangBo Yang BaoYu Chen ShiYun |
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Institution: | 1. Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China 2. Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China;Graduate University of Chinese Academy of Sciences, Beijing 100049, China |
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Abstract: | The aiiA gene from Bacillus thuringiensis was cloned into the Pseudomonas/E. coli shuttle vector and transformed into Pseudomonas aeruginosa strain PAO1. Western blotting showed that the AiiA protein was expressed in PAO1. After induction by IPTG for 6 h and 18
h, expression of the aiiA gene in PAO1 completely degraded the quorum sensing autoinducers N-acylhomoserine lactones (AHLs): N-oxododecanoyl-L-homoserine lactone (OdDHL) and N-butyryl-L-homoserine lactone (BHL). The reduced amount of AHLs in PAO1 was also correlated with decreased expression and
production of several virulence factors such as elastase and pyocyanin. AiiA expression also influenced bacterial swarming
motility. Most importantly, our studies indicated that aiiA played significant roles in P. aeruginosa biofilm formation and dispersion, as observed by the differences of the biofilm formation on liquid and solid surfaces, and
biofilm structures under a scanning electron microscope. |
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Keywords: | Pseudomonas aeruginosa aiiA virulence factors biofilm quorum sensing |
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