Electrophoresis, crossed immunoelectrophoresis, and isoelectric focusing in agarose gels with reduced electroendosmotic flow |
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| Authors: | B G Johansson S Hjertén |
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| Institution: | 1. Department of Clinical Chemistry, University Hospital, S-221 85 Lund Sweden;2. Institute of Biochemistry, University of Uppsala, Uppsala, Sweden |
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| Abstract: | An ion exchange method using QAE-Sephadex for preparation of agarose with a low electroendosmotic flow and reduced adsorption properties is described. The successful use of such agarose for the separation of highly cationic proteins is illustrated.A method for isoelectric focusing of proteins in gels made from a mixture of purified agarose and a water-soluble non-cross-linked acrylamide polymer is described. This method can be combined with immunochemical identification by electrophoresis of the separated components into antibody-containing agarose gels, also containing such a polymer of acrylamide. |
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