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Automation of somatic embryo production
Authors:Ibaraki  Yasuomi  Kurata  Kenji
Affiliation:(1) Department of Biological Science, Yamaguchi University Yoshida, 1677-1, Yamaguchi-shi, Yamaguchi 753-8515, Japan;(2) Department of Biological and Environmental Engineering, The University of Tokyo Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan
Abstract:Automation could enhance the use of somatic embryogenesis for micropropagation in two ways: as effective tools for research on somatic embryogenesis, and for improving the efficiency of embryo production by reducing labor costs. Processes expected to be automated for somatic embryo production are: (1) evaluation of embryogenic cultures, (2) embryo development, (3) harvesting, (4) post-harvesting (pre-delivery) processes for enhancing conversion and preparing for delivery. In this review, the techniques related to the automation of each process are introduced and discussed. This revised version was published online in June 2006 with corrections to the Cover Date.
Keywords:automation  bioreactor  conversion  embryo sorting  image analysis  micropropagation  quality evaluation  somatic embryogenesis
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