Production and characterization of alpha-galactosidase from Aspergillus flavipes |
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Authors: | Ozsoy Nurten Berkkan Hakan |
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Affiliation: | Department of Biochemistry, Faculty of Pharmacy, Istanbul University, 34452 Beyazit, Istanbul, Turkey. |
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Abstract: | An extracellular alpha-galactosidase from the culture filtrate of Aspergillus flavipes grown on melibiose as a carbon source was partially purified by hydroxylapatite and diethylaminoethylcellulose chromatographies. Electrophoretic analysis showed protein bands corresponding to alpha-galactosidase and invertase activities. The optimum pH and temperature were determined as 4.5-5.0 and 45 degrees C, respectively. The Km value for p-nitrophenyl-alpha-d-galactopyranoside was found to be 1.89 mm. The results reported in this study indicate that Aspergillus flavipes is indeed an active source of extracellular alpha-galactosidase. |
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Keywords: | Aspergillus flavipes α‐galactosidase characterization |
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