| 北京鸭红细胞铜锌超氧化物歧化酶电荷异构体的分离和某些性质 |
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| 引用本文： | 李平卫,徐长法,王楠,李根培.北京鸭红细胞铜锌超氧化物歧化酶电荷异构体的分离和某些性质[J].中国生物化学与分子生物学报,1995,11(3):276-280. |
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| 作者姓名： | 李平卫 徐长法 王楠 李根培 |
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| 作者单位： | 北京大学生命科学学院 |
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| 摘 要： | 等电聚焦表明，北京鸭红细胞铜锌超氧化物歧化酶由等电点分别为５．０，５．３，５．９，６．１和６．５的五个主要的活性组分（电荷异构体）构成，利用分析型聚丙烯酰胺凝胶等电聚焦电泳进行电荷异构体的制备级分离，采用三氯乙酸沉淀法快速确定蛋白条带的位置，电渗洗脱法回收蛋白，获得其中两个电荷异构体，对比研究结果表明电荷异构体的活性，氨基酸组成，二级结构等性质无明显差异。
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| 关 键 词： | 北京鸭红细胞 铜锌超氧化物歧化酶 电荷异构体 等电聚焦 圆二色谱 二级结构 |
| 收稿时间： | 1995-06-20 |
Purification and Some Properties of Charge Isomers of Copper and Zinc Superoxide Dismutase from Peking Duck Erythrocytes |
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| Li Ping-Wei,Xu Chang-Fa,Wang Nan,Li Gen-Pei.Purification and Some Properties of Charge Isomers of Copper and Zinc Superoxide Dismutase from Peking Duck Erythrocytes[J].Chinese Journal of Biochemistry and Molecular Biology,1995,11(3):276-280. |
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| Authors: | Li Ping-Wei Xu Chang-Fa Wang Nan Li Gen-Pei |
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| Institution: | (College of Life Sciences,and Department of Chemistry,Peking University, Beijing 100871 |
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| Abstract: | Result of isoelectric focusing electrophoresis shows that copper and zinc superoxide dismutase from Peking duck erythrocytes contains five major active components with isoelectric points at PH5. 0,5. 3,5. 9,6. 1,6. 5 respectively. Two of the major components with PI5.3 and 5.9 were successfully purified by preparative isoelectric focusing method in polyacrylamide gel.The specific activity,amino acid composition,CD spectrum and secondary structure of these two components are nearly the same. |
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| Keywords: | Peking duck erythrocyte Copper and zinc superoxide dismutase Charge isomers Isoelectric focusing CD spectrum Secondary structure |
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