| 同源序列法克隆植物抗病基因研究进展及其在野生稻中的应用 |
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| 引用本文： | 李美玲,严成其,王栩鸣,杨勇,余初浪,周洁,程晔,陈剑平.同源序列法克隆植物抗病基因研究进展及其在野生稻中的应用[J].生物技术通讯,2010,21(1):126-130. |
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| 作者姓名： | 李美玲 严成其 王栩鸣 杨勇 余初浪 周洁 程晔 陈剑平 |
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| 作者单位： | 1. 浙江师范大学,化学与生命科学学院,浙江,金华,321004;浙江省农业科学院,病毒学与生物技术研究所,浙江,杭州,310021
2. 浙江省农业科学院,病毒学与生物技术研究所,浙江,杭州,310021 |
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| 基金项目： | 国家高技术研究发展计划，浙江省自然科学基金，浙江省农业优先项目 |
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| 摘 要： | 随着各种测序计划的完成及生物信息学的发展,植物抗病基因的克隆取得了很大进展,至今有几十个基因已被克隆。研究发现,大多抗病基因都存在特异的保守序列,如富亮氨酸重复序列、核苷酸结合位点、丝氨酸-苏氨酸激酶等。抗病基因的结构特征不仅预示了植物抗病反应中可能的作用机制,而且为分离克隆植物抗病基因提供了一条可行的新途径,如基于同源序列的候选基因法,又称为同源序列法。我们简要综述了已克隆抗病基因的结构、同源序列法克隆抗病基因的研究进展,并对其在野生稻中的应用做了展望。
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| 关 键 词： | 抗病基因 克隆 同源序列法 野生稻 |
Research Advance of Homology-Based Candidate Gene Method and its Application in Wild Rice |
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| LI Mei-ling,YAN Cheng-Qi,WANG Xu-Ming,YANG Yong,YU Chu-Lang,ZHOU Jie,CHENG Ye,CHEN Jian-Ping.Research Advance of Homology-Based Candidate Gene Method and its Application in Wild Rice[J].Letters in Biotechnology,2010,21(1):126-130. |
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| Authors: | LI Mei-ling YAN Cheng-Qi WANG Xu-Ming YANG Yong YU Chu-Lang ZHOU Jie CHENG Ye CHEN Jian-Ping |
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| Institution: | LI Mei-ling1,2,YAN Cheng-Qi2,WANG Xu-Ming2,YANG Yong2,YU Chu-Lang2,ZHOU Jie2,CHENG Ye2,CHEN Jian-Ping2 1.College of Chemistry , Life Science,Zhejiang Normal University,Jinhua 321004,2.Insistitute of Virology , Biotechnology,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China |
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| Abstract: | With the development of molecular biology techniques and bioinformatics,nucleotide sequence of some plant species have been completely determined,and cloning of plant disease resistant genes has been made a great progress.Dozens of disease resistance genes have been cloned.Related studies showed that most of the genes have conserved motifs,such as leucine-rich repeat,nucleotide binding site,serine/threonine kinase etc.The structural characterization of disease resistance genes not only helped us in understa... |
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| Keywords: | disease resistance gene gene cloning homologous sequences wild rice |
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