Expression of CKS1At in Arabidopsis thaliana indicates a role for the protein in both the mitotic and the endoreduplication cycle |
| |
| Authors: | Annie Jacqmard Lieven De Veylder Gerda Segers Janice Almeida Engler Georges Bernier Marc Van Montagu Dirk Inze |
| |
| Institution: | (1) Laboratoire de Physiologie Végétale, Université de Liège, Sart-Tilman, B-4000 Liège, Belgium, BE;(2) Laboratorium voor Genetica, Department of Genetics, Flanders Interuniversity Institute for Biotechnology, Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium, BE;(3) Laboratoire Associé de l'Institut National de 1a Recherche Agronomique (France), Universiteit Gent, B-9000 Gent, Belgium, BE |
| |
| Abstract: | Although endoreduplication is common in plants, little is known about the mechanisms regulating this process. Here, we report
the patterns of endoreduplication at the cellular level in the shoot apex of Arabidopsis thaliana L. Heynh. plants grown under short-day conditions. We show that polyploidy is developmentally established in the pith, maturing
leaves, and stipules. To investigate the role of the cell cycle genes CDC2aAt, CDC2bAt, CYCB1;1, and CKS1At in the process of endoreduplication, in-situ hybridizations were performed on the vegetative shoot apices. Expression of
CDC2aAt, CDC2bAt, and CYCB1;1 was restricted to mitotically dividing cells. In contrast, CKS1At expression was present in both mitotic and endoreduplicating tissues. Our data indicate that CDC2aAt, CDC2bAt, and CYCB1;1
only operate during mitotic divisions, whereas CKS1At may play a role in both the mitotic and endoreduplication cycle.
Received: 11 May 1998 / Accepted: 29 September 1998 |
| |
| Keywords: | :Arabidopsis (cell cycle) CKS1At expression Endoreduplication Meristem Mitotic cycle |
| 本文献已被 PubMed SpringerLink 等数据库收录！ |
|
