The separation of RNA polymerases I and II achieved by fractionation of plant chromatin

Chromatin-bound RNA polymerase I and II from soybean hypocotyl can be separated by differential centrifugation. While all detectable RNA polymerase I is pelleted in association with chromatin, nearly all of the RNA polymerase II activity is not pelleted even at high speeds. Substitution of pH 6 for pH 8 isolation medium results in several-fold greater recovery of chromatin-bound RNA polymerase II.