Evaluation of p-naphtholbenzein-beta-D-galactoside as a substrate for bacterial beta-galactosidase |
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| Authors: | James A L Chilvers K F Perry J D Armstrong L Gould F K |
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| Institution: | Department of Chemical & Life Sciences, University of Northumbria, Newcastle-upon-Tyne, United Kingdom. |
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| Abstract: | We describe the synthesis of a new substrate for the detection of beta-galactosidase and evaluate its performance in comparison with 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal) and cyclohexenoesculetinbeta-D-galactoside (CHE-Gal). Of 206 Enterobacteriaceae strains able to hydrolyze X-Gal, 194 (94.2%) hydrolyzed CHE-Gal and 192 (93.2%) hydrolyzed p-naphtholbenzein-beta-D-galactoside (PNB-Gal). We conclude that PNB-Gal is an effective substrate for the detection of beta-galactosidase. |
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