表达PVY和PLRV双价外壳蛋白基因马铃薯的抗病性研究

表达马铃薯Y病毒(PVY)和马铃薯卷叶病毒(PLRV)双价外壳蛋白基因的马铃薯(Solanum tubero-sum L.)栽培品种“Favorita”和“虎头”,经摩擦接种PVY和用桃蚜接种PLRV后,观察症状并用ELISA测定病毒滴度。结果表明,两个品种转双价CP基因的各株系,接种病毒后表现无症状或症状轻微,其中PVY和PLRV平均滴度均较不转基因对照植株低。不同品种对PVY和PLRV的抗性比较表明,转双价CP基因的“Favorita”对PVY抗性较明显,而转双价CP基因的“虎头”则对PLRV抗性较对PVY抗性明显。不同转基因株系抗病毒水平不同。“Favorita”9个转双价CP基因株系中有6个株系PVY滴度较未转基因对照降低52.5％～90.0％,而“虎头”7个转双价CP基因株系中有4个株系PLRV含量较对照降低53.0％～98.0％。在抗性株系中还出现一些抗1种病毒或抗2种病毒的抗性较强的单株。

RESISTANCE TO MIXED PVY AND PLRV INFECTION IN POTATO CULTIVARS EXPRESSING DUAL PVY AND PLRV COAT PROTEIN GENES

Transgenic clones of potato (Solatium tuberoswn L.) cultivars "Favorita" and "Tiger head" expressing dual potato virus Y (PVY) and potato leaf roll virus (PLRV) coat protein genes were inoculated with PVY and PLRV either by mechanical rubbing or by viruliferoux Myzus persicae Sulz. After inoculation the symptom development was observed and the virus liter was detected wilh DAS-ELISA. Resulls showed that the transgenic clones displayed slight or no symptom. The average PVY and PLRV liters in the transgenic clones were lower than the untransformed plants. Six of the 9 "Favorita" transgenic clones had reduced average PVY tilers were about 52.5%~90.0% lower than that in untransformed plants. Similarly, 4 of the 7 "Tiger head" transgenic clones had reduced average PLRV titers 53.0% ~ 98.0% lower than that in the untransformed plants. Four transgenic clones of "Favorita" and 3 transgenic clones of "Tiger head" were resistant to both PVY and PLRV infection. Some highly resistant to PVY and PLRV transgenic plants were obtained in both transgenic cultivars.